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     The serum reaction was tested with the same emulsions twice afterwards,
namely, in the middle of the fifth week and towards the end of the sixth week.
The result, the same on both occasions, was as follows:—

     Blood was taken from the spleen towards the end of the sixth week.
M. Melitensis was isolated in pure culture (Ferozepore II).

     Before passing on to record those cases in which no splenic puncture was
made and in which the diagnosis rested mainly on the serum agglutination reac-
tion, we have to draw attention to two of the tests, to which the microbes
isolated from the cases described above were subjected.

     We have already pointed out in passing that one of the tests, to which all
the strains confirmed, was the serum agglutination test. This test was carried
out in the following manner:—

     Two rabbits were treated with intra-peritoneal injections of sterile emulsions
of M. Melitensis, one with the race known as stock and the other with the
organism isolated from Sher Hussain (Multan I). The former strain was
procured about two years ago from the Royal Army Medical College, London,
and probably had its origin from a case at Netley Hospital. After several
injections the sera of these rabbits were tested against all the races which had
been isolated by us. The following tables show the results obtained:—

     Table I with the serum of the rabbit immunised with stock M. Melitensis.

     Table II with the serum of the rabbit immunised with M. Melitensis,
          Multan I.