Medicine - Institutions > Army health reports and medical documents > Scientific memoirs by officers of the Medical and Sanitary Departments of the Government of India > Number 17 - Snake-venoms in relation to haemolysis > Snake-venoms in relation to haemolysis
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cause complete hæmolysis when serum complement is present than when it is
absent.
In Table III are recorded the results of the experiments with the same
venoms, dog's washed cells and lecithin as activating agent. From this table it
is seen that all venoms have the power, more or less, of acting as amboceptors
for these cells and free lecithin, but that this power varies considerably in degree,
the poison of Crotalus adamanteus being so weak that even when a large amount
is used complete hæmolysis does not take place. In other respects the table
explains itself and requires no further comment.
Table III.
Amount of venom in milligrammes. |
DOG'S WASHED CELLS: 1 C.C. OF 5 PER CENT. SUSPENSION. LECITHIN: 0.2 C.C. OF 0.1 PER CENT. SOLUTION. |
|||||||
N. T. V. | N. B. V. | B. C. V. | B. F. V. | E. V. V. | D. R. V. | E. C. V. | C. A. V. | |
5 | C. H. | C. H. | C. H. | Well marked. |
C. H. | C. H. | C. H. | Well marked. |
1 | C. H. | Nearly C. H. |
C. H. | C. H. | Nearly C. H. |
C. H. | C. H. | Marked |
0.5 | C. H. | Marked | C. H. | C. H. | N early C. H. |
C. H. | C. H. | Slight |
0.1 | C. H. | Slight | C. H. | C. H. | Trace | C. H. | C. H. | Trace |
0.05 | C. H. | Trace | C. H. | C. H. | Trace | C. H. | Nearly C. H. |
Mere trace |
0.01 | Nearly C. H, |
Trace | C. H. | C. H. | Mere trace. |
C. H. | Marked. | No H. |
0.005 | Well marked. |
No H. | Marked | Nearly C. H. |
No H. | C. H. | Trace | No H. |
0.001 | Slight | No H. | Mere trace. |
Trace | No H. | Well marked. |
No H. | No H. |
0.0005 | Sight | No H. | No H. | No H. | No H. | Slight | No H. | No H. |
C. H.= Complete hæmolysis.
The next observations were made with the red cells of the ox. In this
case not one of the venoms used had any action either alone or when fresh
ox serum was added. It is unnecessary, therefore, to publish the details of
these experiments. The details of the observations when free lecithin was used
as activating agent are given in table IV. From this table it is seen that the
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