Medicine - Institutions > Army health reports and medical documents > Scientific memoirs by officers of the Medical and Sanitary Departments of the Government of India > Number 5 - Specificity of antivenomous sera > Specificity of antivenomous sera
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SERIES No. VI.—Experiments to ascertain if the serum of a horse immunised
with the venom of Hopiocephalus curtus has the power to neutralise the
venom of Bungarus fasciatus.
The same serum as was used in the experiments tabulated in Series No. V was
employed. The observations were made on rabbits. The test dose of venom
was the same as was used in the experiments of Series No. II A, viz., 4 milli-
grammes per kilo., that is to say, about 6 lethal doses. In each instance this
test dose (1 per cent. solution) and the serum in different quantities were mixed
in vitro.
The mixture was allowed to stand at laboratory temperature for half an
hour. In was then injected into the marginal vein of the ear of the rabbit.
The following were the results obtained:—
Animal. | Weight. | Amount of venom per kilo. |
Amount of serum. |
Result. |
Rabbit 1 | 1,450 grammes | 4 milligrammes | 0.5 c. c. | Died in 32 minutes. |
„ 2 | 1,780 ,, | 4 ,, | 1 c. c. | ,, ,, 12 ,, |
„ 3 | 1,460 ,, | 4 ,, | 2 c. c. | ,, ,, 22 ,, |
„ 4 | 1,68o „ | 4 ,, | 5 c. c. | ,, ,, 10 ,, |
„ 5 | 1,490 ,, | 4 ,, | Nil. | ,, ,, 30 ,, |
(Control.) |
From this series of experiments it is at once evident that the serum of a
horse immunised with the venom, of Hoplocephalus curtus has no power to neu-
tralise the venom of Bungarus fasciatus.
SERIES NO. VII.—Experiments to ascertain if the serum of a horse immunised
with the venom of Hoplocephalus curtus has the power to neutralise the
venom of Daboia Russellii.
The serum which was used for these experiments was the same as was
employed for the experiments tabulated in Series Nos. V and VI. Rabbits were
the animals used.
Two series of experiments were performed.
A. In the first series of observations the test dose of venom employed was
0.5 milligramme per kilo., viz., an amount which, as regards this sample of
venom, did not cause intravascular thrombosis when injected intravenously.
This test dose and varying quantities of serum were mixed in vitro. The
mixture was allowed to stand at laboratory temperature for half an hour.
It was then injected into the Marginal vein of the ear of the rabbit.
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