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Scientific Memoirs by

injected into the opposite leg, and four more injections of 0.5 C.C. were similarly
administered during the course of the next five hours.

    Symptoms of intoxication did not appear until the following day, and were
then only slightly marked, and on the next day complete recovery had
occurred.

   Experiment VIII. —A fowl received a minimal lethal dose of cobra-venom
in the right leg, and twenty minutes later a dose of 1 C.C. of solution of anti-
venene in the left one. Four more doses of 0.5 C.C. were administered in the
left leg during the course of the next three hours.

   Symptoms of intoxication began to appear about three hours after the
beginning of the experiment. They continued to be present during the course
of the following day, but were never profound, and on the third day recovery
had evidently set in and progressed steadily.

   Experiment IX. —A fowl received an injection of a minimal lethal dose of
cobra-venom in the right leg, and twenty minutes later one of 1 C.C. of solution
of antivenene, followed by four more doses of 0.5 C.C. during the following two
hours and a half in the left leg.

   Symptoms of intoxication gradually developed and became profound
during the course of the following day, and death occurred about 30 hours after
the beginning of the experiment.

   This series of experiments, in which minimal lethal doses of venom were
followed by treatment with antivenene, seem clearly to indicate the superior
antidotal properties of the dry over the liquid form of the material, for we here
have recovery occurring in four cases out of five, whereas in the case of the
corresponding experiments with the liquid preparation, recovery only followed
in one out of four. The difference in the results is no doubt to be ascribed to
changes having occurred in the liquid preparation during transit to this country,
as Mr. Hankin has demonstrated that, in certain cases, the liquid contains an
abundance of schizomycete organisms, and in some has even a distinctly putre-
factive odour on arrival.

(c ) EXPERIMENTS IN WHICH THE INJECTION OF DOUBLE MINIMAL LETHAL
DOSES OF COBRA-VENOM WAS FOLLOWED BY TREATMENT WITH
SOLUTION OF DRY ANTIVENENE.

   Experiment X. —A fowl received a double minimal lethal dose of cobra-
venom. Twenty minutes later an injection of 1 C.C. of solution was admin-
istered in the same site, and was followed by five more doses of 0.5 C.C. within
the course of the following four hours.

   Symptoms of intoxication began to appear about two and a half hours after
the injection of the venom, and progressed steadily and normally until they
terminated in death about seven hours later.