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                                                  Appendix H.

                                                RINDERPEST.

            Instructions for Inoculating: Bovines with Anti-Rinderpest Serum,

    Manufactured at the Imperial Bacteriological Laboratory, Muktesar,

                                        By "Serum Alone" Method.

                                                SERUM ALONE.

                                    N. B.No virulent blood to be used.

1. The " Serum Alone" method is to be used only in actual outbreaks of Rinder-
pest. It serves to protect animals from contracting the disease and thereby curtails
the spread of the infection. The immunity conferred by a single dose of serum is of
short duration, varying from 3 to 6 weeks. Consequently, if cattle are subjected to
infection for a longer period they should be re-inoculated.

2. The serum is injected subcataneously in the region of the shoulder and, for
this purpose, the hair should be clipped over a small area of about 2 inches diameter,
and the part washed with a 5 per cent. carbolic solution before introducting the needle
of the syringe. After injecting the fluid withdraw the needle placing one finger or thumb
over the small tumour made by the injected serum in order to diffuse the fluid downwards
into the surrounding tissues before letting the animal loose.

After inoculation with serum alone, all the animals should be turned out together with
the infected herd in order that an opportunity of contracting natural infection and a longer
immunity may be given.

Bullocks may be worked on the day following the serum injection.

3. STANDARDISED DOSE OF SERUM—

4.  Each brew of serum is tested before issue on cattle of Hill breed which are most
susceptible to Rinderpest.

The protective standard dose is the amount of serum necessary to protect a Hill bull
weighing 600 lbs. against a simultaneous inoculation of a lethal dose of virulent blood.

No brew of serum is issued which does not protect at a dose of 90 c. c. per 600 lbs.
body weight of Hill cattle.

5.  For plains cattle the dose of serum cannot be standardised owing to the varying
susceptibility of cattle throughout India.

A dose of 5 c.c. per 600 lbs. body weight has been found sufficient to protect village
cattle of the lowest susceptibility.

Doses from 10 c.c. upwards are required according to the susceptibility of the cattle
as evinced by the severity of the outbreak. It remains the duty of the Veterinary Officers
in charge of the operations to regulate the dose of serum for the cattle of their district.

The following instructions have been formulated with a view of assisting in regulating
the dose of serum for plains cattle.

        (i) Doses of 10 to 30 c.c. per 600 lbs. body weight should be used under the
            following circumstances:—

                (a) When from observation of the mortality among non-treated animals there
                is evidence of a high scale of susceptibility.

                (b) When the outbreak is widespread and it is probable that animals will be
                exposed to infection for a long period.