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244           HÆMORRHAGIC SEPTICÆMIA.

                                      BULLS.
                     Serum and virus after 48 hours.

No.

Dose of
Serum.

Dose of
Virus.

Results.

Plains Bulls 1

5 c.c.

0.1 c.c.

Died in 50 hours.

„ „2

10 „

0.1 „

Lived. No reaction.

„ „ 3

10 „

0.1 „

„ „ „

„ „4

15 „

0.1 „

„ Temperature reaction.

„ „5

20 „

0.1 „

„ No reaction.

One Control died in 40 hours.

10 c.c. of this serum was protective against the virus
inoculated 48 hours after.

                                 BUFFALOES.
Serum and virus after 48 hours.

No.

Dose of
Serum.

Dose of
Virus.

Results.

Plains Buffaloes 1

20 c.c.

0.1 c.c.

Lived.

No reaction.

2

25 „

0.1 „

3

25 „

0.1 „

„ „

One Control died in 30 hours.

The serum in a dose of 20 c.c. protects buffaloes against
the virus inoculated 48 hours after.

                           BUFFALOES.
Serum, plus virus after four weeks.

No.

Dose of
Serum.

Dose of
Virus.

Results.

Plains Buffaloes 1

5 c.c.

0.1 c.c.

Died 24 hours.

2

5 „

0.1 „

Lived. No reaction.

3

10 „

0.1 „

„ „ „

4

10 „

0.1 „

Died 30 hours.

5

25 „

0.1 „

Lived. No reaction.

6

25 „

0.1 „

„ „ „

7

25 „

0.1 „

„ „ „

8

25 „

0.1 „

„ „ „

9

25 „

0.1 „

„ „

Two Controls died within 30 hours.

The serum in a dose varying from 10 to 25 c.c. confers
an immunity for a period of about four weeks,

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