PREPARATION OF ANTI-RINDERPEST SERUM.      233

In the course of the preparation of these seras it was
noticed that the fluids diluted with potassium citrate were,
without exception, exceedingly rapidly absorbed. Twenty-four
hours after injection only a thickening remained and this
disappeared after another 24 hours.

Hill Bulls were used throughout, both for providing the
virulent material and for making the seras. Four bulls were
used for making each of the seras B, D and E ; three were used
for making Serum C and two bulls for preparing Serum A.

Serum A.—

Two injections of virulent Peritoneal Washings.

Two Bulls used. 5370—Age 4 years—Weight 266 lbs.
                          5371— „ 2 „ — „ 178 „
Immunised with V.B., and Serum, November 26th, 1910.
Injections. 5370 Injected with 1500 c.c. Peritoneal Washings, December 21st, 1910.
Injected with 2000 c.c. Peritoneal Washings, January 16th, 1911.
Bled 1200 c.c, February 3rd, 1911.
5371. Injected with 1500 c.c. Peritoneal Washings, December 20th, 1910.
Injected with 1800 c.c. Peritoneal Washings, January 12th, 1911.
Bled 1200 c.c., February 1911.
Serum from 5370 and 5371, mixed and tested.

Results of Serum Testings. (A).

First Series—

H. T. R. High Temperature Reaction.
V. B. Virulent Blood.
F. & M. Foot and Mouth Disease.
P. F. Peritoneal Fluid.