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68                  Hæmorrhagic Septicæmia.

                  1 Control died in 36 hours.

Bulls 1, 2, 3, 4 were re-tested a month after the vaccine
injection and inoculation of virus and were found immune.

The investigation of this vaccine was not carried any fur-
ther, as the difficulty in obtaining the pleural exudate proved
that it was not a practical method of operation.

     IMMUNISATION BY MEANS OF TOXIN.

The toxin was obtained by sterilizing and filtering broth
cultures 48 hours old. The toxin was used fresh.

Bull.

Testin.

Tested after

Result.

1

5 c.c.

21 days

Lived

No reaction

2

5 „

21 „

3

5 „

21 „

4

5 „

21 „

5

5 „

42 days

Died.

6

5 „

42 „

7

5 „

12 „

8

5 „

42 „

9

10 „

12 „

10

10 „

42 „

11

10 „

42 „

12

10 „

42 „

The toxin in a dose of 5 c.c. protects for a period of al out
three weeks.

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