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                                        Hæmorrhagic Septicæmia.                                63

Injected with 30 c.c. filtered culture 48 hrs. old.

Interval 12 days.

Injected with 200 c.c. filtered culture 48 hrs. old.

Interval 14 days.

Injected with 600 c.c. filtered culture 48 hrs. old.

Interval 14 days.

Injected with 2000 c.c. filtered culture 48 hrs. old.

Interval 21 days.

Bled 1850 c.c.

Two other hill bulls were treated in a similar manner and
the serum from all three mixed together.

                                            TESTS OF SERUM D.

Serum plus virus 48 hours after. Re-tested after 8 weeks.

Bull.

Dose of
serum in-
jected c.c.

Virus c.c.

Result.

Re-tested
after inter-
val of

Result.

1

5

0.1

Lived. No reaction.

8 weeks.

Lived. No reaction.

2

5

0.1

„ „

„ „

3

10

0.1

„ „

„ „

4

10

0.1

„ „

„ „

                                                  2 Controls died.

Serum alone tested after 6 weeks.

Bull No.

Dose of serum
injected c.c.

Virus c.c.

Result.

1

25

0.1

Lived.

No reaction.

2

25

0.1

3

25

0.1

4

25

0.1

5

25

0.1

Died.

6

25

0.1

Died.

                        3 Controls died ; 1 lived.

Virus followed by serum 24 hours after.

Bull No.

Virus c.c.

Dose of serum
injected c.c.

How injected.

Result.

1

0.1

25

Intrav.

Died 2½ days.

2

0.1

50

Died 3 days.

3

0.1

50

Died 2 days.

                                     2 Controls died.

Serum D appeared somewhat more potent than serum C.
Out of six bulls which received a dose of 25 c.c. and were tested
after an interval of six weeks, four were protected.

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