40                          Charbon Symptomatique.

during process of manipulation renders them little suitable
for use in India.

In every country the success of an anti-serum or vaccine
depends upon simplicity of operation, freedom from accidents
and period of immunity conferred. In India, the first two quali-
fications have to be considered somewhat at the expense of the
third. New methods are regarded with suspicion by the owners
of cattle, and it requires the utmost tact and diplomacy to
induce them to submit their animals for treatment.

To the Hindoo, the life of the cow, as a rule, ranks higher
than that of a human being. Consequently any measures of
treatment adopted for their benefit must, above all considera-
tions, be free from any risk of accidents.

The following experiments were carried out in order to
determine the method of vaccination safest and most easy
of application to the cattle of this country.

In all the following experiments the same virus was used.
This consisted of the dried muscle juice taken from a bull dead
of Black Quarter.

The dried powder was diluted in proportion of 0.2 grammes
in 5 c.c. of normal salt solution. Of this solution 1 c.c. was
determined to be a reliable lethal dose for all susceptible bovines.

Susceptibility of Indian Bovines to experimental Inoculation of

                                      Black Quarter.

Effect of virus inoculated intramuscularly.

The above animals were used as Controls in the experi-
ments testing the vaccines.