V. R. GOPALAKRISHNAN                                  55

Experiments were conducted in Bombay to try " Intravenous alone "
method for natural cases of equine surra. The dose used was 50 c.c. of 10
per cent solution per 1000 lb. body-weight given at intervals of 10 days.
Satisfactory cure was reported after a period of 3 weeks and no relapse oc-
curred even after 2 years in some cases under observation.

Taylor [1934] found the combined method of treatment of Naganol and
tartar emetic to be effective. The routine method of treating equine surra
consisted of a course of seven intravenous injections at intervals of 4 to 5
days in the following dosage:—

The percentage of cure of approximately 87.6 has been reported. The
period of treatment extends to about a month. On an average four and
half gm. of Naganol are required along with seven gm. of tartar emetic to
effect a complete cure. This method has been recommended as it is com-
paratively cheap.

Pfaff [1934] carried out experiments with the use of Antimosan and
Naganol to determine their toxic, curative and prophylactic value. The
results obtained by using Antimosan were very unsatisfactory. On the
other hand, experiments with Naganol gave encouraging results. He suggest-
ed that a single injection of 0.4 gm. Naganol per 100 lb. body-weight would
cure many cases of surra and the same dose repeated on the eighth day
would cure nearly all cases of surra. For prophylaxis the same dose should be
repeated every six weeks.

Shahi [1935-36] adopted a modified technique of four injections con-
sisting of two of 2 per cent Naganol solution at an interval of 22 days and
in between these were sandwiched two injections of 60 to 100 c.c. of 1 per cent
tartar emetic solution once a week. Cases of relapse in mules in some centres
of treatment were reported.

Serum therapy in trypanosomiasis did not show any desirable effect
and hence did not warrant any further work.

                 METHOD OF TREATMENT IN ASSAM

Extensive trials have been made in Assam to regulate and determine
the safe and effective dose of Naganol. The earliest attempts at treatment
with various chemotherapeutic agents are of less importance as compared
with the success that attended the standardization of the dosage of the present
routine method that has been found most suitable to the field conditions of
Assam. It was on the 5 July 1935 that an experimental attempt was made to
work out the dose of Naganol and the necessary period that should elapse
between the injections. The Director, Veterinary Department, Assam,
Rai Sahib Srish Chandra Ghose, initiated these experiments and it was under
his instruction and guidance that a satisfactory method was evolved. During