344 THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY [ I, IV

The first animal used in this series of experiments was a grade Holstein cow.
This animal withstood the intravenous injection of 400 cc of 20 per cent, sodium
citrate solution. Although this did produce decided muscular tremors, the animal
remained on its feet. The sodium citrate solution was injected by means of a 40-cc
syringe over a period of twenty minutes. No calcium chlorid was administered to
this animal.

                                                 TABLE II.

Summary of experiments with intravenous injection of sodium citrate into cows of
                                                  dairy lype.

Two grade Shorthorn cows were subsequently killed by the rapid injection,
under pressure, of 800 and 600 cc respectively, of sodium citrate solution. Both
animals failed to respond to the injection of 800 cc of a 20 per cent, solution of
calcium chlorid.

Another grade Shorthorn cow which was to be used for anatomy dissection
was given 640 cc of the sodium citrate solution into the jugular vein by means of
a 40-cc syringe over a period of 32 minutes at which time the animal went down.
This was preceded by a period of excitement, muscular tremors and inco-ordination.
There was also a marked expiratory dyspnea present. This animal was allowed to
remain in the recumbent position without being given the calcium chlorid treat-
ment. Forty-five minutes later, the animal was urged to get up but walked with
a decidedly stilted gait and showed signs of tetany and inco-ordination. The ani-
mal was again put down by the intravenous injection of 160 cc of the sodium,
citrate solution. The animal was then bled out, preparatory to embalming. A
sample of blood was collected from this animal which was only partially coagulated