FOWL TYPHOID IN INDIA                          105

         It is thus not possible, as has already been reported by a number of workers, to
distinguish by the agglutination test between fowl typhoid caused by S. gallinarum
and bacillary white diarrhœa caused by S. pullorum.

         Dead bacterial culture was prepared for use as a vaccine, by growing the
organism on agar plates, collecting the growth, emulsifying it in carbolised normal
saline solution to opacity 8 by barium sulphate standard tubes and heating it at
65°C. for one hour. This vaccine, however, failed entirely to protect fowls in-
oculated with it against a small dose of the living organism given by subcutaneous
injection. Details are given in Table IV.

                                             TABLE IV.