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adult only. A third has to be added for I shall show that in the case of R.
sanguineus nymphs of the second generation may be infective.

       It is obvious that the explanation of these facts was difficult for, up to quite
recently, when a few of the stages in development were described by Koch and
Kleine, not a single trustworthy observation existed regarding the development
of piroplasma in the tick. Even the work of Koch and Kleine left the nature of
the changes undergone nearly as obscure as before, since the significance of the
stages described by these authors was not at all clear and at most their obser-
vations formed only a few disconnected links in the chain of events.

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